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Cellular damages caused by cryopreservation in trout sperm.

Author(s) : HERRAEZ M. P., MEDIAVILLA M., ALVAREZ R., SANCHEZ A. J., MANSO A., PAZ P. de

Summary

Brown trout sperm were cryopreserved in a programmable biocooler using two different extenders. Motility, vitality, fertility and ultrastructure were evaluated before and after freezing. Thirty minutes after dilution in all the diluents, plasma membrane structure was damaged, and sperm cells diluted in glycerol media showed major mitochondrial alterations. After freezing, the percentage of cell lysis increased, all structural damages became widespread. A preliminary study of the membrane glucoconjugates was carried out using labelled lectins for fluorescence microscopy. Use of lectins as a tool for the study of fish sperm membrane integrity is also discussed.

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Details

  • Original title: Cellular damages caused by cryopreservation in trout sperm.
  • Record ID : 1997-0971
  • Languages: English
  • Source: Refrigeration and Aquaculture.
  • Publication date: 1996/03/20
  • Document available for consultation in the library of the IIR headquarters only.

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