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Flow cytometric analysis of plasma membrane damages of rainbow trout and turbot frozen spermatozoa.

Analyse par cytométrie en flux des dommages membranaires des spermatozoïdes congelés de truite arc-en-ciel et de turbot.

Author(s) : OGIER DE BAULNY B., LE VERN Y., KERBOEUF D., HEYDORFF M., MAISSE G.

Summary

The aim of the study was to understand the difference in freeze-thawing survival between trout and turbot spermatozoa by evaluating membrane damage of spermatozoa. Flow cytometric analyses were performed on cryopreserved turbot and trout spermatozoa after fluorescent staining with propidium iodide to assess plasma membrane integrity. The composition of the freezing medium used is given. These penetrating cryoprotectants were tested. The freezing rate was -4/minute until -80 deg C, followed by storage at -196 deg C and thawing to 4 deg C. The results, suggesting different cryoprotectant-membrane interactions in trout and turbot, are discussed in light of membrane composition of spermatozoa of each species.

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Details

  • Original title: Analyse par cytométrie en flux des dommages membranaires des spermatozoïdes congelés de truite arc-en-ciel et de turbot.
  • Record ID : 1997-0972
  • Languages: French
  • Source: Refrigeration and Aquaculture.
  • Publication date: 1996/03/20
  • Document available for consultation in the library of the IIR headquarters only.

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