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Cryopreservation of neomale rainbow trout milts.

La cryoconservation de laitance de salmonidés et plus spécifiquement de néomales de truite arc-en-ciel.

Author(s) : FELLER E., DEMOL T., DENIS P.

Summary

To facilitate the raising of 100%-female rainbow trout, without having to produce neomales, the authors developed a technique of cryopreservation of milts that is useable in the field. Ten thousand ovums can thus be fertilized with fifty to sixty 0.5-ml straws, with a fertility rate of 70%. The minimum quantity of thawed to be used to fertilize is about three million. The freezing viability of the soft roe varies among males (10 to 30%) and for the same male the period of spermiation varies (also 10 to 30%). It was essential to develop a method of selecting of these soft roe. Three methods were compared: 1) microscopic observation to determine the percentage of motility of the thawed spermatozoa; 2) determination of the percentage of transminase oxaloacetate glutamate (TOG) released into the cryoconservation environment, with the developement of a calibration curve; and 3) determining the percentage of DNA released by drawing up a calibration curve. In conclusion, percentage of mobility must be higher than 20%, whereas the percentage of TOG should be lower than 40% in order to make cryopreservation of milts worthwhile. The released DNA is not dosable through spectrophotometry in the cryopreservation laitance diluter mixture.

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Details

  • Original title: La cryoconservation de laitance de salmonidés et plus spécifiquement de néomales de truite arc-en-ciel.
  • Record ID : 1997-0970
  • Languages: French
  • Source: Refrigeration and Aquaculture.
  • Publication date: 1996/03/20
  • Document available for consultation in the library of the IIR headquarters only.

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