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COOLING RATE/CULTURE MEDIUM INTERACTIONS IN THE SURVIVAL AND STRUCTURAL STABILITY OF CRYOPRESERVED SHOOT-TIPS OF BRASSICA NAPUS.

Author(s) : WITHERS L. A., BENSON E. E., MARTIN M.

Type of article: Article

Summary

CULTURED SHOOT-TIPS OF BRASSICA NAPUS CAN SURVIVE CRYOPRESERVATION BY EITHER A RAPID OR A SLOW RATE OF COOLING. THE FREQUENCY AND STRUCTURAL PATTERN OF RECOVERY GROWTH ARE HIGHLY DEPENDENT UPON THE CULTURE MEDIUM USED IN THE RECOVERY PHASE. A MEDIUM CONTAINING THE CYTOKININ 6-BENZYLAMINOPURINE PROMOTES ORGANISED RECOVERY VIA NORMAL SHOOT GROWTH BUT ONLY AFTER RAPID COOLING, WHEREAS A MEDIUM CONTAINING THE SYNTHETIC AUXIN 2,4-D AND COCONUT WATER SUPPORTS RECOVERY VIA CALLUS FORMATION AFTER BOTH COOLING REGIMES, SHOOT REGENERATION OCCURRING RARELY. ON THE LATTER MEDIUM, RAPID COOLING STILL YIELDS A HIGHER FREQUENCY OF RECOVERY.

Details

  • Original title: COOLING RATE/CULTURE MEDIUM INTERACTIONS IN THE SURVIVAL AND STRUCTURAL STABILITY OF CRYOPRESERVED SHOOT-TIPS OF BRASSICA NAPUS.
  • Record ID : 1989-0125
  • Languages: English
  • Source: Cryo-Letters - vol. 9 - n. 2
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

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