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Cryopreservation of in vitro-grown shoot tips of tea (Camellia sinensis) by vitrification.

Author(s) : KURANUKI Y., SAKAI A.

Type of article: Article

Summary

Two-month-old plantlets of tea were cold-hardened for more than 5 weeks. Then the shoot tips (2 mm in length) of terminal buds were excised and precultured for 2 days at 5 deg C. Cold-hardened and precultured meristems were dehydrated by a highly concentrated vitrification solution (PVS2) for 90 minutes at 0 deg C prior to a direct plunge into liquid nitrogen. Successfully vitrified and warmed meristems resumed growth within 5 days and directly developed shoots without intermediary callus formation. The average rate of normal shoot formation was 60% about 1 month after planting. The shoot formation of meristems cooled to -196 deg C by encapsulation/dehydration technique was 40%. Thus, the vitrification procedure appears promising as a routine method for cryopreserving meristems of tea plant.

Details

  • Original title: Cryopreservation of in vitro-grown shoot tips of tea (Camellia sinensis) by vitrification.
  • Record ID : 1996-3465
  • Languages: English
  • Source: Cryo-Letters - vol. 16 - n. 6
  • Publication date: 1995/11
  • Document available for consultation in the library of the IIR headquarters only.

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