Share

Cryopreservation of in vitro-grown shoot tips of mulberry by vitrification.

Author(s) : NIINO T., SAKAI A., ENOMOTO S., MAGOSI J., KATO S.

Type of article: Article

Summary

Three-week-old in vitro mulberry plantlets were cold-hardened at 5 deg C for 3 weeks. Excised shoot tips from hardened plantlets were precultured on agar-Murashige and Skoog medium supplemented with 0.7 M sucrose for 1 day at 5 deg C. Shoot tips were transferred to a 2 ml plastic cryotube and a highly concentrated cryoprotective solution (vitrification solution) was then added. After treatment with this solution at 25 deg C for 90 min, the shoot tips were directly plunged into liquid nitrogen. Successfully vitrified and warmed shoot tips regained green colour in about 4 days after plating and developed shoots within 10 days. The average rate of normal shoot formation was about 65%. Thus, this vitrification method appears promising as a routine method for cryopreserving apical meristem of in vitro mulberry plants.

Details

  • Original title: Cryopreservation of in vitro-grown shoot tips of mulberry by vitrification.
  • Record ID : 1994-0938
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 5
  • Publication date: 1992/09
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (6)
See the source

Indexing