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Evolution of the solute concentration in biological models submitted to an isochoric high pressure process.

Author(s) : SANZ P. D., GUIGNON B., APARICIO C., et al.

Summary

The high pressure is, at least, as effective as the cryoprotectors are for decreasing both the homogenous nucleation and the freezing temperatures. This fact allows for the storage of biological material without icing-up at lower temperatures than at atmospheric pressure. Moreover, the characteristics of ice (size, distribution, location) produced under pressure make it less harmful for the biological tissues than the one obtained at atmospheric pressure. The cryopreservation by both isobaric and isochoric processes is possible at high pressures. Different fluids are used as biological models but, unlike pure water, very few data related with their phase diagram under pressure are available. It is due to the lack of suitable equipment and to the difficulty to carry out these measurements. In this work, the phase transition curves for sodium chloride and for sucrose solutions are given as a function of the concentration, for different pressures from 0.1 to about 150-210 MPa. The parameters of a polinomial equation are fit to experimental points. So the solute concentration can be calculated at any temperature and pressure equilibrium position during the freezing of the model solution. Simulating the cryoconcentration during a cryopreservation process by high pressure freezing, damages due to solution, osmotic, toxic effects could be evaluated.

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Pages: ICR07-C1-525

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Details

  • Original title: Evolution of the solute concentration in biological models submitted to an isochoric high pressure process.
  • Record ID : 2008-0937
  • Languages: English
  • Source: ICR 2007. Refrigeration Creates the Future. Proceedings of the 22nd IIR International Congress of Refrigeration.
  • Publication date: 2007/08/21

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