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Impact of DMSO concentrations on cell death markers and viability in cryopreserved human keratinocytes.

Number: 0023

Author(s) : CECHOVA K., STIBRANIOVA I., GALFIOVA P., BALCERZAK L., KLBIK I., WACZULIKOVA I., LIBUSA S.

Summary

Cryopreservation of cells is a critical challenge in cryobiology and tissue engineering. The cryopreservation method affects the quality of cells after thawing, as cells are susceptible to freeze-thaw stress, leading to damage or death. Therefore, cryoprotective agents are used to protect cells during cryopreservation. This study investigates the effect of cryopreservation on human keratinocytes using dimethyl sulfoxide (DMSO) as a cryoprotectant. We evaluated low (1.8% and 2.2%) and standard (5% and 10%) DMSO concentrations during short-term storage at −80°C. After thawing, we monitored cell viability, plasma membrane fluidity, and signs of cell death using electron microscopy. Lower DMSO concentrations significantly reduced keratinocyte viability due to increased apoptotic activity and stress, while higher concentrations provided better protection and maintained higher viability. Membrane fluidity increased with higher DMSO concentrations, which facilitates apoptotic signaling. Morphological and ultrastructural analyses revealed significant changes at lower DMSO concentrations and nuclear damage at higher concentrations. These findings have implications for improving.cryopreservation protocols to enhance cell viability and functionality after thawing.

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Pages: 7 p.

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Details

  • Original title: Impact of DMSO concentrations on cell death markers and viability in cryopreserved human keratinocytes.
  • Record ID : 30033880
  • Languages: English
  • Subject: Technology
  • Source: Cryogenics 2025. Proceedings of the 18th IIR International Conference on Cryogenics, Prague, Czech Republic, 7-11 April 2025.
  • Publication date: 2025/04/07
  • DOI: http://dx.doi.org/10.18462/iir.cryo.2025.0023

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