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Cryopreservation of cell suspensions of Citrus deliciosa Tan and histological study.

Author(s) : AGUILAR M. E., ENGELMANN F., MICHAUX-FERRIERE N.

Type of article: Article

Summary

Optimal conditions for the cryopreservation of cell suspensions of Citrus deliciosa Tan were defined. After a one-hour cryoprotective treatment with 0.15 M sucrose and 5% DMSO, cells were prefrozen to -40 deg C at 0.5 deg C/min and immersed in liquid nitrogen. After rapid thawing, survival rates were between 50 and 60% of unfrozen controls. Proliferation in liquid medium was optimal after a 5-day recovery period on solid medium. Production of somatic embryos was higher with cryopreserved cultures than with unfrozen control cultures.

Details

  • Original title: Cryopreservation of cell suspensions of Citrus deliciosa Tan and histological study.
  • Record ID : 1994-0937
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 4
  • Publication date: 1993/07
  • Available in the IIR library

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