Cryopreservation of cell suspensions of Citrus deliciosa Tan and histological study.
Author(s) : AGUILAR M. E., ENGELMANN F., MICHAUX-FERRIERE N.
Type of article: Article
Summary
Optimal conditions for the cryopreservation of cell suspensions of Citrus deliciosa Tan were defined. After a one-hour cryoprotective treatment with 0.15 M sucrose and 5% DMSO, cells were prefrozen to -40 deg C at 0.5 deg C/min and immersed in liquid nitrogen. After rapid thawing, survival rates were between 50 and 60% of unfrozen controls. Proliferation in liquid medium was optimal after a 5-day recovery period on solid medium. Production of somatic embryos was higher with cryopreserved cultures than with unfrozen control cultures.
Details
- Original title: Cryopreservation of cell suspensions of Citrus deliciosa Tan and histological study.
- Record ID : 1994-0937
- Languages: English
- Source: Cryo-Letters - vol. 14 - n. 4
- Publication date: 1993/07
- Document available for consultation in the library of the IIR headquarters only.
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Indexing
- Themes: Seeds and plants
- Keywords: Thawing; Suspension; Plant (botany); Cryoprotectant; Citrus fruit; Cell; Liquid nitrogen; DMSO; Survival; Sucrose; Embryo; Cryopreservation; Freezing
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Advantages of cryopreservation of cell suspensi...
- Author(s) : ENGELMANN F., AGUILAR M. E., DAMBIER D., CABASSON C., MICHAUX-FERRIERE N., OLLITRAULT P.
- Date : 1994/01
- Languages : English
- Source: Fruits - vol. 49 - n. 1
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Cryopreservation of grape embryogenic cell susp...
- Author(s) : DUSSERT S., et al.
- Date : 1991/09
- Languages : English
- Source: Cryo-Letters - vol. 12 - n. 5
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Effects of pregrowth and freezing conditions on...
- Author(s) : BAGNIOL S., ENGELMANN F.
- Date : 1991/09
- Languages : English
- Source: Cryo-Letters - vol. 12 - n. 5
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Vitrification of Oryza sativa L. cell suspensions.
- Author(s) : WATANABE K., STEPONKUS P. L.
- Date : 1995/09
- Languages : English
- Source: Cryo-Letters - vol. 16 - n. 5
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CRYOPRESERVATION OF MUSA SUSPENSION CULTURES AN...
- Author(s) : PANIS B. J., WITHERS L. A., LANGHE E. A. L. de
- Date : 1990
- Languages : English
- Source: Cryo-Letters - vol. 11 - n. 5
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