Effects of pregrowth and freezing conditions on the resistance of meristems of date palm (Phoenix dactylifera L. var. Bou Sthammi Noir) to freezing in liquid nitrogen.

Type of article: Article

Summary

Optimal cryoprotection and freezing conditions were studied for date palm meristems. A 1-or 3-day culture period on standard medium, immediately after their dissection, prior to pregrowth on medium containing 0.5 M sucrose, was necessary in order to obtain the survival of meristems after freezing in liquid nitrogen. The maximal survival was reached after cryoprotection with 0.5 M sucrose and 5 to 15% dimethylsulfoxide (DMSO), followed by freezing at 1 deg C/min to -30 deg C before immersion in liquid nitrogen.

Details

Original title: Effects of pregrowth and freezing conditions on the resistance of meristems of date palm (Phoenix dactylifera L. var. Bou Sthammi Noir) to freezing in liquid nitrogen.
Record ID : 1993-0269
Languages: English
Source: Cryo-Letters - vol. 12 - n. 5
Publication date: 1991/09
Document available for consultation in the library of the IIR headquarters only.

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Indexing

Themes: Seeds and plants
Keywords: Tree (plant); Plant (botany); Cryoprotectant; Liquid nitrogen; DMSO; Survival; Sucrose; Horticulture; Date; Cryopreservation