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Effects of pregrowth and freezing conditions on the resistance of meristems of date palm (Phoenix dactylifera L. var. Bou Sthammi Noir) to freezing in liquid nitrogen.

Author(s) : BAGNIOL S., ENGELMANN F.

Type of article: Article

Summary

Optimal cryoprotection and freezing conditions were studied for date palm meristems. A 1-or 3-day culture period on standard medium, immediately after their dissection, prior to pregrowth on medium containing 0.5 M sucrose, was necessary in order to obtain the survival of meristems after freezing in liquid nitrogen. The maximal survival was reached after cryoprotection with 0.5 M sucrose and 5 to 15% dimethylsulfoxide (DMSO), followed by freezing at 1 deg C/min to -30 deg C before immersion in liquid nitrogen.

Details

  • Original title: Effects of pregrowth and freezing conditions on the resistance of meristems of date palm (Phoenix dactylifera L. var. Bou Sthammi Noir) to freezing in liquid nitrogen.
  • Record ID : 1993-0269
  • Languages: English
  • Source: Cryo-Letters - vol. 12 - n. 5
  • Publication date: 1991/09
  • Document available for consultation in the library of the IIR headquarters only.

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