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Vitrification of Oryza sativa L. cell suspensions.

Author(s) : WATANABE K., STEPONKUS P. L.

Type of article: Article

Summary

Cells were loaded with a 1.5 M DMSO solution at 20 deg C for 20 minutes, and then dehydrated with a vitrification solution of 44.5 wt% DMSO + 18.7 wt% sorbitol at 0 deg C for 4 minutes. After quenching in liquid nitrogen, the cells were warmed and unloaded in a 0.5 M sorbitol solution at 20 deg C for 30 minutes. A few colonies were formed during the post-thaw culture. The cell suspension resulting from the colonies grew in the same manner as the original cell suspension.

Details

  • Original title: Vitrification of Oryza sativa L. cell suspensions.
  • Record ID : 1996-2271
  • Languages: English
  • Source: Cryo-Letters - vol. 16 - n. 5
  • Publication date: 1995/09
  • Document available for consultation in the library of the IIR headquarters only.

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