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Cryopreservation of porcine blastocysts by vitrification.

Author(s) : YOSHINO J., KOJIMA T., SHIMIZU M., TOMIZUKA T.

Type of article: Article

Summary

The objective of the study was to assess the possibility of cryopreservation of porcine expanded and hatched blastocysts by vitrification. The following four types of vitrification solutions were applied in this study: 1) EFT, a mixture of 7.2 M ethylene glycol, 0.003 M ficoll, and 0.3 M trehalose; 2) DAP213, 2 M dimethyl sulfoxide, 1 M acetamide, and 3 M propylene glycol; 3) DAP213-T, DAP213 supplemented with 0.3 M trehalose; and 4) EPT, 4 M ethylene glycol, 3.1 M propylene glycol and 0.3 M trehalose. As a whole, the viabilities of embryos equilibrated in the solutions were lower than those of control embryos. The stepwise equilibration employed was superior to a single-step equilibration in the in vitro survival of, especially, expanded blastocysts after dilution. In the vitrification test, the embryos were equilibrated in the solutions by the four-step method, loaded into 0.25-milliliter plastic straw, and plunged into liquid nitrogen.

Details

  • Original title: Cryopreservation of porcine blastocysts by vitrification.
  • Record ID : 1994-1897
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 4
  • Publication date: 1993/08

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