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RAPID CRYOPRESERVATION OF SHEEP EMBRYOS BY DIRECT TRANSFER INTO LIQUID NITROGEN VAPOUR AT 93 K (-180 DEG C).

Author(s) : SZELL A., ZHANG J., HUDSON R.

Type of article: Article

Summary

THE IN VITRO SURVIVAL OF RAPIDLY CRYOPRESERVED SHEEP EMBRYOS WAS EXAMINED AS A FUNCTION OF THE DURATION OF EXPOSURE TO A VITRIFICATION MEDIUM (25% GLYCEROL + 25% PROPYLENE GLYCOL). THE DURATION OF EXPOSURE TO THE VITRIFICATION MEDIUM AT 291-296 K (18-23 DEG C) HAD NO EFFECT ON THE SURVIVAL OF RAPIDLY CRYOPRESERVED EMBRYOS ; AT 277-285 K (4-12 DEG C), HOWEVER, THERE WAS AN EFFECT. OVERALL, THE IN VITRO SURVIVAL RATE OF CONTROL AND CRYOPRESERVED EMBRYOS INCREASED WITH ADVANCING DEVELOPMENT FROM LATE MORULAE (36%) TO LATE BLASTOCYSTS (70%). THESE RESULTS SHOWED THAT SHEEP EMBRYOS CAN BE SUCCESSFULLY CRYOPRESERVED BY A SIMPLE, RAPID PROCEDURE.

Details

  • Original title: RAPID CRYOPRESERVATION OF SHEEP EMBRYOS BY DIRECT TRANSFER INTO LIQUID NITROGEN VAPOUR AT 93 K (-180 DEG C).
  • Record ID : 1991-2788
  • Languages: English
  • Source: Reprod. Fertil. Dev. - vol. 2 - n. 6
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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