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Cryopreservation of specific pathogen-free (SPF) pig islet cells: effect of culture time before cryopreservation and after thawing.

Author(s) : DUVIVIER V., DARQUY S., REACH G.

Type of article: Article

Summary

The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells cryopreserved 7 days after isolation and cultured 3 days after thawing. Islet cells were cryopreserved 1, 7 or 14 days after isolation and cultured 3, 7, 14, or 21 days after thawing. Islet cell number, insulin content, and insulin response under perifusion tests were investigated. It was concluded that SPF pig islet cells remained functional after cryopreservation in polyethylene glycol and it may be important to culture islet cells over 14 days before and after cryopreservation.

Details

  • Original title: Cryopreservation of specific pathogen-free (SPF) pig islet cells: effect of culture time before cryopreservation and after thawing.
  • Record ID : 2000-2233
  • Languages: English
  • Source: Cryobiology - vol. 38 - n. 4
  • Publication date: 1999/06

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