ShareCryopreservation of porcine hepatocyte cultures.
Author(s) : KOEBE H. G., DÄHNHARDT C., MÜLLER-HÖCKER J., WAGNER H., SCHILDBERG F. W.
Type of article: Article
Summary
With cryopreservation of freshly isolated hepatocytes, a dramatic loss in cell number, viability is observed, and differentiated cell function is usually inevitable, and, upon thawing, hepatocytes do not attach well in culture. The authors have compared the performance of porcine hepatocytes from slaughterhouse organs that were frozen. Cryopreservation of hepatocyte cultures could provide a practical means of establishing a bank of hepatocyte cultures. This could be extremely useful for the development of a hybrid artificial liver device.
Details
- Original title: Cryopreservation of porcine hepatocyte cultures.
- Record ID : 1997-0550
- Languages: English
- Source: Cryobiology - vol. 33 - n. 1
- Publication date: 1996/02
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cell; Pork; Cryopreservation; Liver
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Cryohemostasis of uncontrolled haemorrhage from...
- Author(s) : KOPELMAN D., KLEIN Y., ZARITSKY A., et al.
- Date : 2000/05
- Languages : English
- Source: Cryobiology - vol. 40 - n. 3
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Cryopreservation of specific pathogen-free (SPF...
- Author(s) : DUVIVIER V., DARQUY S., REACH G.
- Date : 1999/06
- Languages : English
- Source: Cryobiology - vol. 38 - n. 4
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Subzero osmotic characteristics of intact and d...
- Author(s) : KORNISKI B., DARR T. B., HUBEL A.
- Date : 1999/06
- Languages : English
- Source: Cryobiology - vol. 38 - n. 4
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CRYOGENIC HOMOGENIZATION OF BIOLOGICAL TISSUES.
- Author(s) : LANGLAND J. K.
- Date : 1983
- Languages : English
- Source: NBS spec. Publ. - n. 656
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CRYOPRESERVATION OF ISOLATED HEPATOCYTES.
- Author(s) : FULLER B. D.
- Date : 1988
- Languages : Russian
- Source: Kriobiologija - n. 3
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