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Cryopreservation of Taxus chinensis suspension cell cultures.

Author(s) : KIM S. I., CHOI H. K., SON J. S., et al.

Type of article: Article

Summary

A simple cryopreservation method for suspension cells of Taxus chinensis was established. In this procedure 7 day old suspension cells were used without a preculture treatment. Cells were incubated in cryoprotectant solution (0.5 M DMSO and 0.5 M glycerol) on ice for 30 min and then frozen at a cooling rate of 1 °C/min to -40 °C prior to immersion in liquid nitrogen. The average viability of frozen-thawed cells was between 30 to 40%. The recovery of cryopreserved cells in liquid nitrogen for 1 month was accomplished. After rapid thawing, cells were transferred to solid medium and cultivated for 4-6 weeks. Trehalose treatment enhanced re-growth of frozen-thawed cells. The stable maintenance of paclitaxel biosynthetic ability in cryopreserved cells was confirmed by comparing with that of regularly sub-cultures suspension cells.

Details

  • Original title: Cryopreservation of Taxus chinensis suspension cell cultures.
  • Record ID : 2002-0518
  • Languages: English
  • Source: CryoLetters - vol. 22 - n. 1
  • Publication date: 2001/01
  • Document available for consultation in the library of the IIR headquarters only.

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