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CRYOPRESERVED BONE MARROW CELLS IN HUMAN TRANSPLANTATION: FUNCTIONAL AND CLINICAL RESULTS.
Author(s) : BEAUJEAN F.
Summary
THE BONE MARROW FROM 50 PATIENTS WAS FROZEN IN TWO FORMS: WHOLE CELLS AND BUFFY COAT PREPARED BY DENSITY GRADIENT ON FICOLL (IBM 2991). THE CRYOPROTECTANT IS DMSO AT 10%, COOLING RATE 1 K/MIN, STORAGE IN LIQUID NITROGEN. AFTER THAWING DMSO IS REMOVED. THE RECOVERY OF MARROW CELLS AND STEM CELLS WAS STUDIED IN VITRO. THAWED MARROW WAS TRANSFUSED, AFTER INTENSIVE CHEMOTHERAPY (POSSIBLY FOLLOWED BY TOTAL BODY IRRADIATION). HEMATOLOGICAL RECOVERY AFTER NORMAL TIMES WAS OBSERVED IN ALL CASES.
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Pages: 97-102
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Details
- Original title: CRYOPRESERVED BONE MARROW CELLS IN HUMAN TRANSPLANTATION: FUNCTIONAL AND CLINICAL RESULTS.
- Record ID : 1985-1307
- Languages: English
- Source: [Refrigeration serving humanity]. Proceedings of the XVIth international Congress of Refrigeration.
- Publication date: 1983/08/07
- Document available for consultation in the library of the IIR headquarters only.
Links
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cryoprotectant; Cancer; DMSO; Marrow; Leukemia; Graft; Cryopreservation
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DECLINED VIABILITY AND DIFFERENTIATION OF HUMAN...
- Author(s) : MATSUKI R.
- Date : 1989
- Languages : English
- Source: Low Temperature Medicine - vol. 15 - n. 1
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CRYOPRESERVATION OF BLOOD CELLS.
- Author(s) : HARSANYI V., RACZ Z., THEK M.
- Date : 1987/08/02
- Languages : English
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SEPARATION AND CRYOPRESERVATION OF HUMAN MYELOI...
- Author(s) : MCGANN L. E.
- Date : 1983/08/07
- Languages : English
- Source: [Refrigeration serving humanity]. Proceedings of the XVIth international Congress of Refrigeration.
- Formats : PDF
View record
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STUDIES ON TOXICITY AND APPLICATION DOSE OF CRY...
- Author(s) : LIU Z.
- Date : 1989/10/11
- Languages : English
View record
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STUDIES ON CRYOPRESERVATION OF BONE MARROW.
- Author(s) : LIU Z.
- Date : 1987/08/24
- Languages : English
- Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
- Formats : PDF
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