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Simple and rapid cryopreservation of rat embryos by vitrification.

Author(s) : OHBOSHI S., FUJIHARA N.

Type of article: Article

Summary

Rat blastocyts were vitrified using a solution containing 18.5% DMSO, 14.0% acetamide, 9.0% propylene glycol and 5.4% polyethylene glycol. The embryos were vitrified by two methods. There was no significant difference between the two methods in post-thaw development of the vitrified embryos. The diluents containing sucrose and glycerol were shown to be favourable for removing cryoprotectants from the frozen-thawed embryos.

Details

  • Original title: Simple and rapid cryopreservation of rat embryos by vitrification.
  • Record ID : 1994-1876
  • Languages: English
  • Source: Low Temperature Medicine - vol. 18 - n. 1
  • Publication date: 1992/03
  • Document available for consultation in the library of the IIR headquarters only.

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