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Deep freezing preservation of mammalian spermatozoa.

Author(s) : MASUDA H.

Type of article: Article

Summary

Principal constituents of diluents used for sperm freezing are sugars (glucose, lactose), lipoproteins (egg yolk, milk) and zwitterionic buffers. The optimum levels of these constituents to achieve maximum motility after freezing and thawing vary for sperm of different species. The most commonly used cryoprotective agent is glycerol, but its apparent toxicity also varies for different species. A synthetic detergent and wetting agent is very effective for the prevention of freeze-thaw damage to boar and stallion sperm. The aim of the present review is to document recent developments in the cryopreservation of sperm from bull, boar and stallion.

Details

  • Original title: Deep freezing preservation of mammalian spermatozoa.
  • Record ID : 1997-1835
  • Languages: English
  • Source: Low Temperature Medicine - vol. 22 - n. 2
  • Publication date: 1996/06
  • Document available for consultation in the library of the IIR headquarters only.

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