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DEFORMATION OF THE CELL MEMBRANE AT LOW TEMPERATURES. 1. A CRYOMICROSCOPICAL TECHNIQUE. 2. ELASTICAL DEFORMABILITY OF THE ERYTHROCYTE MEMBRANE.

Author(s) : THOM F., MATTHES G.

Type of article: Article

Summary

CELLS CAN BE POLARIZED IN A HIGH-FREQUENCY ELECTRIC FIELD OF 0.1 LOWER THAN F LOWER THAN 5 MEGAHERTZ. INCREASING THE FIELD STRENGTH UP TO 2 KILOWATTS/CM ELONGATES CELLS WHICH HAVE SURFACE INDICES HIGHER THAN 1. THE CELLS ARE SUSPENDED IN LOW IONIC STRENGTH SOLUTIONS CONTAINING NONIONIC CRYOPROTECTIVE AGENTS. THE SAMPLE WITH GOLD-WIRE ELECTRODES IS MOUNTED ON THE PROGRAMMABLE COLD-STAGE OF A LOW TEMPERATURE LIGHT MICROSCOPE THE RESULTS SHOW A RAPID INCREASE IN THE ELASTIC MODULUS WITH DECREASING TEMPERATURE. SUBSTITUTION OF GLYCEROL IN CRYOPROTECTIVE CONCENTRATION HAS NO INFLUENCE ON THE MECHANICAL BEHAVIOUR. AT NEARLY 253 K (-20 DEG C) THE DEFORMABILITY OF ERYTHROCYTES IS ZERO.

Details

  • Original title: DEFORMATION OF THE CELL MEMBRANE AT LOW TEMPERATURES. 1. A CRYOMICROSCOPICAL TECHNIQUE. 2. ELASTICAL DEFORMABILITY OF THE ERYTHROCYTE MEMBRANE.
  • Record ID : 1989-2075
  • Languages: English
  • Source: Cryo-Letters - vol. 9 - n. 5
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

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