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Determination of water permeability parameters and optimal freezing rates of human red blood cells by differential scanning calorimeter.

[In Chinese. / En chinois.]

Author(s) : ZHOU X., YUAN J., WANG H., et al.

Type of article: Article

Summary

Optimization of human red blood cells (RBCs) cryopreservation protocols requires an understanding of the water permeability characteristics. Volume shrinkage during freezing of RBCs can be obtained by using a shape-independent differential scanning calorimeter (OSC) technique. By fitting the water transport model to the experimentally obtained OSC data, the best-fit membrane permeability parameters (reference membrane permeability to water and the activation energy) in normal saline, 20 % dimethyl sulfoxide (Me2SO), 10% glycerol and 20% glycerol were determined. These parameters were then used to simulate water transport of RBCs at constant cooling rates between O.S-SO'C/min. The simulation results showed "optimal cooling rate" for erythrocytes were S.2'C/min, SSC/min and 4.6'C/min in 20 % Me2SO, 10% and 20% glycerol respectively.

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Pages: 66-69

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