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Differing effects of various cryoprotectants on intercellular junctions of epithelial (Madin-Darby canine kidney) cells.

Author(s) : ARMITAGE W. J., JUSS B. K., EASTY D. L.

Type of article: Article

Summary

At 22 deg C, DMSO had the least effect of the three cryoprotectants, causing a gradual fall in transepithelial resistance of only 17% in 30 minutes. This compares with falls of 50% in 15 minutes and 37% over 30 minutes, respectively, with glycerol and propane-1,2-diol. At 0 deg C, however, propane-1,2-diol had no effect on transepithelial resistance, whereas the effect of DMSO was now similar to that of glycerol, viz. a sustained reduction in resistance of approximatively 30% throughout the 30-minutes exposure.

Details

  • Original title: Differing effects of various cryoprotectants on intercellular junctions of epithelial (Madin-Darby canine kidney) cells.
  • Record ID : 1995-3880
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 1
  • Publication date: 1995/02

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