Effect of antifreeze proteins on the motility of ram spermatozoa.
Author(s) : PAYNE S. R., OLIVER J. E., UPRETI G. C.
Type of article: Article
Summary
The effect of the antifreeze peptide type 1 from winter flounder, Pseudopleuronectes americanus, and the antifreeze glycoprotein from Antarctic cod, Dissostichus mawsoni, was assessed on the motility of ram spermatozoa after chilling (5 deg C) and after freeze-thawing. The effect was not concentration dependent nor did it depend on which antifreeze protein was added. However, due to the cytotoxicity during the chilling stage, only antifreeze peptide at a concentration of 10 microgram/milliter increased the percentage of motile spermatozoa significantly following freezing and thawing over that of the control (P lower than 0.05). Possible mechanisms are proposed.
Details
- Original title: Effect of antifreeze proteins on the motility of ram spermatozoa.
- Record ID : 1995-1223
- Languages: English
- Source: Cryobiology - vol. 31 - n. 2
- Publication date: 1994/04
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Sperm; Ram; Cryoprotectant; Antifreeze; Protein; Survival; Cryopreservation
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