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EFFECT OF CRYOPROTECTANTS ON THE VIABILITY AND FUNCTION OF UNFROZEN HUMAN POLYMORPHONUCLEAR CELLS.

Author(s) : TAKAHASHI T.

Type of article: Article

Summary

HIGH CONCENTRATIONS OF MEMBRANE PERMEABLE CRYOPROTECTANTS ARE NECESSARY TO PROTECT HUMAN POLYMORPHONUCLEAR LEUKOCYTES FROM OSMOTIC STRESS INJURY DURING FREEZING, BUT SOME CRYOPROTECTANTS ARE CHEMICALLY TOXIC. CELLS WERE EXPOSED TO GLYCEROL, DMSO, OR ETHYLENE GLYCOL FOR 5 MIN TO 2 HR AT 310, 295 OR 273 K (37, 22 OR 0 DEG C), ADDING OR REMOVING THE CRYOPROTECTANT EITHER SLOWLY OR RAPIDLY. 1 AND 2 M CONCENTRATIONS GENERALLY ARE NOT TOXIC AT 295 K. ADDITION AND REMOVAL OF GLYCEROL AT 273 K WAS INJURIOUS EVEN AT 1 M. THE TOXICITY CAN BE LARGELY EXPLAINED BY OSMOTIC STRESS OR DILUTION SHOCK RATHER THAN CHEMICAL TOXICITY ; ETHYLENE GLYCOL IS THE EASIEST CRYOPROTECTANT TO ADD TO AND REMOVE FROM THESE CELLS.

Details

  • Original title: EFFECT OF CRYOPROTECTANTS ON THE VIABILITY AND FUNCTION OF UNFROZEN HUMAN POLYMORPHONUCLEAR CELLS.
  • Record ID : 1986-1718
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 4
  • Publication date: 1985

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