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THE EFFECT OF CRYOPROTECTANTS ON ELECTRIC PARAMETERS OF MOUSE EMBRYO CELL MEMBRANES.

[In Russian. / En russe.]

Author(s) : CEKUROVA N. R., KISLOV A. N., VEPRINSEV B. N.

Type of article: Article

Summary

IT IS SHOWN THAT SUCH CRYOPROTECTANTS AS DMSO, ETHYLENE GLYCOL, AND FORMAMIDE DECREASE BOTH THE CELL MEMBRANE PERMEABILITY FOR IONS AND MEMBRANE POTENTIAL. CHANGES IN MEMBRANE ELECTRIC PARAMETERS DEPENDED ON THE CRYOPROTECTANT TYPE AND CONCENTRATION. DMSO AND FORMAMIDE IN CONCENTRATIONS LOWER THAN 5 M DID NOT CHANGE MEMBRANE ELECTRIC PROPERTIES AT ROOM TEMPERATURE WHILE ETHYLENE GLYCOL APPEARED TO EXERT A TOXIC EFFECT IN CONCENTRATIONS HIGHER THAN 3 M. THE FREEZE-THAWING PROCEDURE CHANGED MEMBRANE ELECTRICAL PROPERTIES INDEPENDENTLY OF THE PRESENCEOF CRYOPROTECTANTS. MEMBRANE PARAMETERS OF THAWED CELLS CULTURED FOR 2 HOURS WERE RESTORED IN A MEDIUM CONTAINING CELLULAR METABOLITES.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1990-2609
  • Languages: Russian
  • Source: Kriobiologija - n. 1
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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