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EFFECT OF DISSOLVED GASES ON FREEZE-THAW SURVIVAL OF MAMMALIAN CELLS.

Author(s) : KRUUV J.

Type of article: Article

Summary

DEGASSINGTHE CELLS AND THE MEDIUM BY APPLYING A REDUCED PRESSURE OF 25 TORR FOR 30 MIN AT ROOM TEMPERATURE PRIOR TO A FREEZE-THAW CYCLE RESULTED IN SIGNIFICANTLY HIGHER SURVIVALS AFTER SUCH A CYCLE THAN FOR CONTROLS WHETHER SINGLE CELLS OR MULTI-CELLULAR SPHEROIDS WERE USED. FREEZING SAMPLES IN PLASTIC VIALS GAVE HIGHER SURVIVALS THAN FREEZING IN GLASS VIALS. THE DEGASSING TECHNIQUE IS EFFECTIVE IN INCREASING SURVIVAL IN THE PRESENCE AND ABSENCE OF SERUM AND/OR DMSO.

Details

  • Original title: EFFECT OF DISSOLVED GASES ON FREEZE-THAW SURVIVAL OF MAMMALIAN CELLS.
  • Record ID : 1986-0884
  • Languages: English
  • Source: Cryo-Letters - vol. 6 - n. 4
  • Publication date: 1985
  • Document available for consultation in the library of the IIR headquarters only.

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