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PARAMETERS AFFECTING THE OSMOTIC BEHAVIOR OF CELLS DURING FREEZING AND THAWING: HUMAN GRANULOCYTES.

Author(s) : SCHWARTZ G. J., DILLER K. R.

Type of article: Article

Summary

INDIVIDUAL HUMAN GRANULOCYTES WERE EXAMINED VISUALLY DURING FREEZING AND THAWING USING A CRYOMICROSCOPE. THE FREEZING EVENTS WERE RECORDED BY SEQUENTIAL PHOTOMICROGRAPHY. THE APPARENT TRANSIENT CELL VOLUMES WERE DETERMINED BY DIRECT MEASUREMENT AS A FUNCTION OF TEMPERATURE. CELLS EXHIBITED SENSIVITY IN OSMOTIC RESPONSE TO MANIPULATION OF THE COOLING AND WARMING RATES, THE EXTRACELLULAR NUCLEATION TEMPERATURE, AND THE HOLDING TIME AT THE NUCLEATION STATE.

Details

  • Original title: PARAMETERS AFFECTING THE OSMOTIC BEHAVIOR OF CELLS DURING FREEZING AND THAWING: HUMAN GRANULOCYTES.
  • Record ID : 1982-1408
  • Languages: English
  • Source: Cryo-Letters - vol. 2 - n. 11
  • Publication date: 1981
  • Document available for consultation in the library of the IIR headquarters only.

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