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EFFECTS OF CRYOPROTECTANTS ON ACTIN FILAMENTS DURING THE CRYOPRESERVATION OF ONE-CELL RABBIT EMBRYOS.

Author(s) : VINCENT C.

Type of article: Article

Summary

A DYNAMIC EQUILIBRIUM BETWEEN GLOBULAR AND FILAMENTOUS ACTIN PLAYS A CRUCIAL ROLE IN CELL STRUCTURE AND MOTILITY. MANY FACTORS ARE KNOWN TO INFLUENCE THIS EQUILIBRIUM. SOME ORGANIC SOLVENTS, SUCH AS THOSE USED FOR THE CRYOPRESERVATION OF CELLS, MAY ALSO ALTER THE DYNAMIC EQLIT BIOCHEMICAL TECHNIQUES WERE USED TO STUDY THE INFLUENCE OF SOLVENTS ON RABBIT SKELETAL ACTIN. RESULTS OBTAINED DEMONSTRATE THAT PROPANEDIOL HAS A DUAL EFFECT ON ACTIN POLYMERS IN VITRO: IT DECREASES THE PROPORTION OF FILAMENTOUS ACTIN AND THE REMAINING FILAMENTS APPEAR SHORTER AND AGGREGATE TO FORM BUNDLES. IN CONTRAST, DMSO DOES NOT ALTER DRAMATICALLY THE ACTIN POLYMER INTEGRITY. PROPANEDIOL IS SHOWN TO EXERT A GOOD CRYOPROTECTIVE ACTION ON RABBIT EMBRYOS, WHILE DMSO DOES NOT.

Details

  • Original title: EFFECTS OF CRYOPROTECTANTS ON ACTIN FILAMENTS DURING THE CRYOPRESERVATION OF ONE-CELL RABBIT EMBRYOS.
  • Record ID : 1991-0889
  • Languages: English
  • Source: Cryobiology - vol. 27 - n. 1
  • Publication date: 1990

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