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EFFECT OF RAPID ADDITION AND DILUTION OF DMSO AND 310 K (37 DEG C) EQUILIBRATION ON VIABILITY OF RABBIT MORULAE THAWED RAPIDLY.

Author(s) : KOJIMA T., SOMA T., OGURI N.

Type of article: Article

Summary

THE AIM OF THE STUDY WAS TO EXAMINE THE EFFECTS OF VARIOUS CONDITIONS OF ADDITION AND DILUTION OF DMSO AND 310 K EQUILIBRATION, AND ALSO THE EFFECTS OF FREEZING IN THE SOLUTION WHICH WAS PREPARED IN ADVANCE AND STORED IN PLASTIC STRAWS AT 253 K (-20 DEG C) ON THE VIABILITY OF RABBIT MORULAE THAWED RAPIDLY. THE EMBRYOS WERE COOLED FROM ROOM TEMPERATURE TO 243 K (-30 DEG C) AT 1 K/MIN IN THE PRESENCE OF 1.5 M DMSO USING A PROGRAMMABLE LIQUID NITROGEN VAPOUR FREEZING MACHINE WITH AN AUTOMATIC SEEDING DEVICE THEN COOLED RAPIDLY, AND STORED IN LIQUID NITROGEN. THE FROZEN STRAWS WERE THAWED RAPIDLY (MORE THAN 16.7 K/SEC). WHEN EMBRYOS WERE PIPETTED INTO 1.5 M DMSO THAT WAS PREPARED IN ADVANCE, STOCKED IN STRAWS AT 253 K, AND COOLED, THE PROPORTIONS OF TRANSFERABLE AND DEVELOPED EMBRYOS WERE EQUIVALENT TO THOSE OF EMBRYOS FROZEN IN THE SOLUTION THAT WAS PREPARED IMMEDIATELY BEFORE USE.

Details

  • Original title: EFFECT OF RAPID ADDITION AND DILUTION OF DMSO AND 310 K (37 DEG C) EQUILIBRATION ON VIABILITY OF RABBIT MORULAE THAWED RAPIDLY.
  • Record ID : 1989-0375
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 3
  • Publication date: 1987

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