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Effects of hypothermia on the function, membrane integrity, and cytoskeletal structure of hepatocytes.

Author(s) : STEFANOVICH P., EZZELL R. M., SHEEHAN S. J., TOMPKINS R. G., YARMUSH M. L., TONER M.

Type of article: Article

Summary

The authors characterized the effects of hypothermia on long-term function and cytoskeletal organization of hepatocytes cultured in the collagen sandwich configuration, which maintains the expression of liver-specific functions for several weeks. In these studies, cultured hepatocytes (maintained at 37 deg C for 7 days) were exposed to 4 deg C in Leibovitz-15 (L15), University of Wisconsin solution, or L15 supplemented with 2.5 g% polyethylene glycol (PEG) for various time periods followed by a return to normothermia. PEG may be a useful additive in preservation solutions for hepatocytes in hepatocyte-based liver support systems and for intact tissue as well. Detailed results are given.

Details

  • Original title: Effects of hypothermia on the function, membrane integrity, and cytoskeletal structure of hepatocytes.
  • Record ID : 1996-1873
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 4
  • Publication date: 1995/08

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