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Long-term storage of isolated liver cells at hypothermia.

Author(s) : KRAVCHENKO L., ANDRIYENKO A., BELOUS A., SHANINA I.

Type of article: Article

Summary

The authors report that media containing a non-penetrating component (O.25 M sucrose) and an antioxidant (silibor) are able to preserve rat hepatocytes with a high metabolic status for long periods at 4 deg C. Survival of hepatocytes after normothermic incubation indicated an intact mitochondrial system, both at the stage of isolation and after hypothermic storage. Thus, by inhibiting osmotic damage during hypothermic storage, the authors produced an extended preservation period for isolated liver cells at 4 deg C. The addition of a drug possessing antioxidant properties was also beneficial.

Details

  • Original title: Long-term storage of isolated liver cells at hypothermia.
  • Record ID : 1995-0580
  • Languages: English
  • Source: Cryo-Letters - vol. 15 - n. 3
  • Publication date: 1994/05
  • Document available for consultation in the library of the IIR headquarters only.

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