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Effect of cryoprotectant treatment and post-thaw washing on the survival of cultured rice (Oryza sativa L.) cells after cryopreservation.

Author(s) : WATANABE K., KURIYAMA A., KAWAI F, et al.

Type of article: Article

Summary

Cultured rice cells differing in cryoprotectability were loaded with 5% (v/v) DMSO and 10% (w/v) D-glucose at 0 °C for 1h. Both the sugar content and osmotic pressure of loaded cells became higher than those of untreated cells. In highly cryoprotectable cells, those values were reduced to the level of untreated cells by washing with liquid medium. For the poorly cryoprotectable cells, however, they remained higher than those of the untreated control cells after washing. Two-dimensional electrophoresis of proteins extracted from the cells during a post-thaw culture showed that cell contents were lost from the poorly cryoprotectable cells at the initial stage of post-thaw culture, suggesting that the poorly cryoprotectable cells frozen-thawed are damaged more or less by osmotic stress and do not survive after cryopreservation in liquid nitrogen.

Details

  • Original title: Effect of cryoprotectant treatment and post-thaw washing on the survival of cultured rice (Oryza sativa L.) cells after cryopreservation.
  • Record ID : 2000-3211
  • Languages: English
  • Source: Cryo-Letters - vol. 20 - n. 6
  • Publication date: 1999/11
  • Document available for consultation in the library of the IIR headquarters only.

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