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EFFECTS OF THE TEMPERATURE OF EXTRACELLULAR ICE-SEEDING ON SURVIVAL OF FROZEN AND THAWED MOUSE MORULAE.

Author(s) : MIYAMOTO H., ISHIBASHI T.

Type of article: Article

Summary

MOUSE MORULAE WERE FROZEN TO 77 K (-196 DEG C) IN PRESENCE OF ETHYLENE-GLYCOL AND GLYCEROL. WHEN ICE-SEEDING OCCURRED IN EMBRYOS AT 263 K (-10 DEG C) OR BELOW, IN VITRO SURVIVAL RATES WERE LOWER THAN THOSE OBTAINED WITH ICE-SEEDING AT 269 OR 265 K (-4 OR -8 DEG C) AND THE RATIO OF MORULAE DEVELOPING INTO YOUNG MICE WAS ALSO LOWER. (Bull. CNRS, FR., 81-361-13342.

Details

  • Original title: EFFECTS OF THE TEMPERATURE OF EXTRACELLULAR ICE-SEEDING ON SURVIVAL OF FROZEN AND THAWED MOUSE MORULAE.
  • Record ID : 1982-2119
  • Languages: English
  • Source: Experientia - vol. 37 - n. 2
  • Publication date: 1981

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