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ULTRASTRUCTURE BEFORE FREEZING, WHILE FROZEN, AND AFTER THAWING IN ASSESSING CRYOINJURY OF MOUSE EPIDIDYMAL SPERMATOZOA.

Author(s) : SHERMAN J. K., LIU K. C.

Type of article: Article

Summary

TAILS OF MOUSE EPIDIDYMIDES WERE FROZEN WITH AND WITHOUT CRYOPROTECTIVE AGENT TO BELOW 193 K (-80 DEG C), SLOWLY (8 K/MIN) OR RAPIDLY (18 K/SEC). RESULTS SHOWED NO EVIDENCE OF DELETERIOUS ULTRASTRUCTURAL EFFECTS OF FREEZING AT RATES COMPARED, OR OF BENEFITS OF CRYOPROTECTIVE AGENTS, REGARDLESS OF THEIR CELLULAR LOCATION. DIFFERENCES WERE NOTED IN THE APPEARANCE, AS A FUNCTION OF THE RATE OF FREEZING BUT NOT AS A FUNCTION OF THE PRESENCE OF EITHER GLYCEROL OR DMSO. NO SPERMATOZOA SURVIVED, SHOWING THE LETHAL EFFECT OF BOTH EXTRACELLULAR ICE DURING SLOW FREEZING AND OF INTRACELLULAR AND/OR EXTRACELLULAR ICE DURING RAPID FREEZING.

Details

  • Original title: ULTRASTRUCTURE BEFORE FREEZING, WHILE FROZEN, AND AFTER THAWING IN ASSESSING CRYOINJURY OF MOUSE EPIDIDYMAL SPERMATOZOA.
  • Record ID : 1983-1173
  • Languages: English
  • Source: Cryobiology - vol. 19 - n. 5
  • Publication date: 1982

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