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Endothelial cell detachment from synthetic substrate materials at 4 deg C.

Author(s) : MCCARTY J. P., HUBEL A.

Type of article: Article

Summary

The purpose of the investigation was to quantify the detachment of endothelial cells as a function of time at 4 deg C for various substrates. Human umbilical cord endothelial cells were harvested and plated on gelatin coated substrates of glass, polyester, and nylon. From 0-38 hours of hypothermia, the overall area covered was decreasing and the area per cell was decreasing rapidly with time, but the average number of cells per field was nearly constant. From 40-48 hours of hypothermia, the average number of cells per field was decreasing rapidly, and the average area per cell as a function of time remained constant.

Details

  • Original title: Endothelial cell detachment from synthetic substrate materials at 4 deg C.
  • Record ID : 1997-2481
  • Languages: English
  • Source: Cryo-Letters - vol. 17 - n. 4
  • Publication date: 1996/07
  • Document available for consultation in the library of the IIR headquarters only.

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