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Evaluation of different preparation parameters for the production and cryopreservation of seed cultures with recombinant Saccharomyces cerevisae.

Author(s) : SCHU P., REITH M.

Type of article: Article

Summary

The authors describe the intensive investigation of defined physicochemical parameters for their influence on the preparation, storage stability in liquid nitrogen, and the post-thaw productivity of a recombinant strain of Saccharomyces cerevisiae expressing the human factor XIIIa protein. The viability of the cells after reconstitution of the frozen batches was found to improve significantly if a certain combination of prethaw growth conditions was applied and amino acids were included in the cryoprotectant media. In addition, the influence of different concentrations of glycerol as a cryoprotectant was investigated and the specific freezing conditions which improved the viability were identified. Even without complex freezing devices for the controlled freezing of cultures, viability rates of 85% and higher were obtained, enabling consistent production of the recombinant protein.

Details

  • Original title: Evaluation of different preparation parameters for the production and cryopreservation of seed cultures with recombinant Saccharomyces cerevisae.
  • Record ID : 1996-1874
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 4
  • Publication date: 1995/08

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