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Experimental study on thermal properties of porcine kidney added glycerol as cryoprotective agent.

[In Chinese. / En chinois.]

Author(s) : AN N., ZHU K., WANG Y., et al.

Type of article: Article

Summary

In order to preserve porcine kidney at cryobiological relevant temperatures, the effects of different concentrations of glycerol on the freezing point, apparent specific heat, the latent heat of porcine kidney were investigated between -80~20? by using Differential Scanning Calorimeter (DSC). Thermal constant Analyzer (Hot Disk) was used to measure the thermal conductivity of porcine kidney in varying mass fraction of glycerol. Results show that as the mass fraction of glycerol increases, the thawing phase transition range of porcine kidney shift to the lower temperature range, the freezing point and the apparent latent heat both decrease. The change of apparent specific heat can be divided into three phases. Before the phase change, apparent specific heat increase with the increases of mass fraction of glycerol; in phase-change region, apparent specific heat is significantly reduced as the mass fraction of glycerol increases; and after the phase change, apparent specific heat are lower for higher glycerol mass fraction. And the thermal conductivity goes down as glycerol mass fraction increases. The thermal characteristics can provide theoretical basis for organ cryopreservation.

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Pages: 66-69

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