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EXPRESSION AND LOCALIZATION OF BACTERIAL LUCIFERASE DETERMINED BY IMMUNOGOLD LABELLING.

Author(s) : COLEPICOLO P.

Type of article: Periodical article

Summary

USING A POLYCLONAL ANTIBODY RAISED AGAINST PURIFIED LUCIFERASE OF VIBRIO HARVEYI AND IMMUNOGOLD LABELLING ON THIN SECTIONS, THE AMOUNTS AND CELLULAR LOCALIZATION OF LUCIFERASE WERE EXAMINED DURING THE GROWTH OF THE BACTERIA. CELLS HARVESTED AT DIFFERENT TIMES DURING CULTIVATION IN LIQUID MEDIUM AT 295 K (22 DEG C) WERE FIXED, EITHER CHEMICALLY OR BY FAST FREEZE FIXATION FOLLOWED BY FREEZE SUBSTITUTION, AND EMBEDDED IN LR WHITE. CONCOMITANT MEASURES OF BIOLUMINESCENCE, BOTH IN VITRO AND IN VIVO, SHOWED THE CLASSICAL CURVE OF AUTOINDUCTION. THE NUMBER OF GOLD PARTICLES PER CELL AREA SHOWED A SIMILAR PATTERN. THEIR LOCALIZATION WAS ALWAYS CYTOPLASMIC.

Details

  • Original title: EXPRESSION AND LOCALIZATION OF BACTERIAL LUCIFERASE DETERMINED BY IMMUNOGOLD LABELLING.
  • Record ID : 1991-0417
  • Languages: English
  • Source: Arch. Microbiol. - n. 152
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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