ShareSummary
FREEZING OF WHOLE CELLS WITH LIQUID NITROGEN INDUCED DECREASE OF RESPIRATORY ACTIVITY TO UNDER 20% OF THAT OF ORIGINAL CELLS. MITOCHONDRIA HARVESTED FROM FREEZE-THAWED CELLS HAVE MARKEDLY DECREASED SUCCINATE OXIDIZING ACTIVITY. ACTIVITY OF SUCCINATE CYTOCHROME C REDUCTASE WAS REDUCED SIGNIFICANTLY WHILE ACTIVITIES OF SUCCINATE DEHYDROGENASE AND CYTOCHROME C OXIDASE WERE REDUCED SLIGHTLY. THE ACTIVITIES OF SUCCINATE OXIDATION IN MITOCHONDRIA FROM FREEZE-THAWED CELLS WERE RESTORED TO NORMAL LEVELS BY THE ADDITION OF CYTOCHROME C. IT WAS CONCLUDED THAT THE DECREASED RESPIRATORY ACTIVITIES OF YEAST CELLS CAN BE ATTRIBUTED PRIMARILY TO THE ELUTION OF CYTOCHROME C FROM MITOCHONDRIA.
Details
- Original title: FREEZING INJURY IN THE YEAST RESPIRATORY SYSTEM.
- Record ID : 1986-2537
- Languages: English
- Source: Cryobiology - vol. 23 - n. 1
- Publication date: 1986
Links
See other articles in this issue (6)
See the source
Indexing
-
The freeze-thaw stress response of the yeast Sa...
- Author(s) : PARK J. I., GRANT C. M., ATTFIELD P. V., DAWES I. W.
- Date : 1997/10
- Languages : English
- Source: Appl. environ. Microbiol. - vol. 63 - n. 10
View record
-
FREEZING INJURY OF MALATE DEHYDROGENASES BY 2-M...
- Author(s) : DOM-ENECH C.
- Date : 1987
- Languages : English
- Source: Cryo-Letters - vol. 8 - n. 2
View record
-
On the nature of nonlethal cryoinjuries in yeasts.
- Author(s) : TSUTSAYEVA A. A., KUDOKOTSEVA O. V.
- Date : 1992/09/10
- Languages : English
- Source: Cryo-Letters - vol. 13 - n. 5
View record
-
THE ACTIVITY OF SOME ENZYMES AS A MARKER OF MEM...
- Author(s) : IVANOV N., et al.
- Date : 1979/08/27
- Languages : English
View record
-
FREEZING AND THAWING INDUCED CURING OF DRUG-RES...
- Author(s) : CALCOTT P., WOOD D., ANDERSON L.
- Date : 1983
- Languages : English
- Source: Cryo-Letters - vol. 4 - n. 2
View record