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FREEZING INJURY IN THE YEAST RESPIRATORY SYSTEM.

Author(s) : MORI Y., SUZUKI H., NEI T.

Type of article: Article

Summary

FREEZING OF WHOLE CELLS WITH LIQUID NITROGEN INDUCED DECREASE OF RESPIRATORY ACTIVITY TO UNDER 20% OF THAT OF ORIGINAL CELLS. MITOCHONDRIA HARVESTED FROM FREEZE-THAWED CELLS HAVE MARKEDLY DECREASED SUCCINATE OXIDIZING ACTIVITY. ACTIVITY OF SUCCINATE CYTOCHROME C REDUCTASE WAS REDUCED SIGNIFICANTLY WHILE ACTIVITIES OF SUCCINATE DEHYDROGENASE AND CYTOCHROME C OXIDASE WERE REDUCED SLIGHTLY. THE ACTIVITIES OF SUCCINATE OXIDATION IN MITOCHONDRIA FROM FREEZE-THAWED CELLS WERE RESTORED TO NORMAL LEVELS BY THE ADDITION OF CYTOCHROME C. IT WAS CONCLUDED THAT THE DECREASED RESPIRATORY ACTIVITIES OF YEAST CELLS CAN BE ATTRIBUTED PRIMARILY TO THE ELUTION OF CYTOCHROME C FROM MITOCHONDRIA.

Details

  • Original title: FREEZING INJURY IN THE YEAST RESPIRATORY SYSTEM.
  • Record ID : 1986-2537
  • Languages: English
  • Source: Cryobiology - vol. 23 - n. 1
  • Publication date: 1986

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