Share

IMMUNOGOLD LABELLING OF LUCIFERASE IN THE LUMINOUS BACTERIUM VIBRIO HARVEYI AFTER FAST-FREEZE FIXATION AND DIFFERENT FREEZE-SUBSTITUTION AND EMBEDDING PROCEDURES.

Author(s) : NICOLAS M. T., BASSOT J. M., NICOLAS G.

Type of article: Article

Summary

THE AUTHORSSTUDIED THE ULTRASTRUCTURAL LOCALIZATION OF LUCIFE RASE ON SECTIONS OF THE BIOLUMINESCENT BACTERIUM VIBRIO HARVEYI BY INDIRECT IMMUNOGOLD STAINING, USING A POLYCLONAL ANTILUCIFERASE ANTIBODY AND THE USUAL CONTROL TESTS, AFTER CHEMICAL FIXATION OR FAST-FREEZE FIXATION FOLLOWED BY DIFFERENT FREEZE-SUBSTITUTION PROCEDURES AND EMBEDDING IN EITHER E LR WHITE. LABELLING INTENSITY WAS 3.7 TIMES GREATER IN LR WHITE THAN IN EPON. WITH BOTH RESINS, LABELLING DIMINISHED SIMILARLY WHEN FIXATIVE AGENTS WERE PRESENT IN TH E-T. THE LOCALIZATION OF LUCIFERASE IN THE CYTOPLASM AND PARTICULARLY IN THE PATCHES IS DISCUSSED.

Details

  • Original title: IMMUNOGOLD LABELLING OF LUCIFERASE IN THE LUMINOUS BACTERIUM VIBRIO HARVEYI AFTER FAST-FREEZE FIXATION AND DIFFERENT FREEZE-SUBSTITUTION AND EMBEDDING PROCEDURES.
  • Record ID : 1991-0416
  • Languages: English
  • Source: J. Histochem. Cytochem. - vol. 37 - n. 5
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

Links


See the source

Indexing