FACTORS OF CRYOINJURY IN BIOLOGICAL MEMBRANES ON SLOW FREEZING. INVESTIGATION OF ERYTHROCYTE GHOSTS BY THE METHOD OF NMR RELAXATION.

[In Russian. / En russe.]

Author(s) : SAHAROV B. V., VOLKOV V. Ja.

Type of article: Article

Summary

THE INFLUENCE OF UNFROZEN WATER AND DISSOLVED IONS ON THE STABILITY OF MEMBRANES WAS STUDIED BY THE METHOD OF NMR RELAXATION DURING FREEZING OF ERYTHROCYTE GHOSTS RECONSTITUTED USING CHOLINE CHLORIDE AND POTASSIUM CHLORIDE SOLUTIONS OF DIFFERENT OSMOLARITY. THE RESULTS INDICATED DECREASING THE LEVEL OF UNFROZEN WATER IN THE SYSTEM BELOW THE CRITICAL VALUE OF 5-4.1 TO BE THE MAIN FACTOR WHICH INDUCES A NON-SPECIFIC IMPAIRMENT OF THE ERYTHROCYTE MEMBRANE PERMEABILITY UPON SLOW FREEZING. SALT PRECIPITATION FROM THE UNFROZEN PHASE MAY BE AN ALTERNATIVE CAUSE OF THE MEMBRANE DAMAGE.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1990-0411
  • Languages: Russian
  • Source: Kriobiologija - n. 3
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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