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Freeze-thaw injury in living cells.

Author(s) : FARRANT J.

Type of article: Article, IJR article

Summary

Preservation of mammalian systems is reviewed with special reference to tissue culture cells, lymphocytes and embryos. It was found that cell shrinkage during cooling is necessary for preservation. Both cooling rate and two-step methods allowed this cell shrinkage to occur without the excessive imposition of other damaging factors. Insufficient cell shrinkage during cooling caused the formation of excessive amounts of intracellular ice that may damage the ceils during rewarming. Conversively in some systems, notably embryos, the presence of a small amount of intracellular ice may aid survival after rapid thawing, Illustrations are provided in support of two final points. Firstly, all variables involved in preservation procedures interact; secondly functional assays of thawed material may require different conditions to those worked out for unfrozen controls.

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Pages: 191-195

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Details

  • Original title: Freeze-thaw injury in living cells.
  • Record ID : 30000895
  • Languages: English
  • Source: International Journal of Refrigeration - Revue Internationale du Froid - vol. 3 - n. 4
  • Publication date: 1980/07

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