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PROTECTIVE EFFECT OF L-GLUTAMINE AGAINST FREEZE-THAW DAMAGE IN MAMMALIAN CELLS.

Author(s) : KRUUV J., GLOFCHESKI D. J., LEPOCK J. R.

Type of article: Article

Summary

L-GLUTAMINE AT 18 MILLIMOLES PROTECTS MAMMALIAN CELLS AGAINST FREEZE-THAW (FT) DAMAGE BY A FACTOR OF ABOUT 6 IN BALANCED SALT SOLUTIONS. WHILE NOT NEARLY AS EFFECTIVEAS DMSO OR PROPYLENE GLYCOL (PG), THE MECHANISM OF PROTECTION BY GLUTAMINE APPEARS TO BE INDEPENDENT FROM THAT OF DMSO OR PG. THERE IS NO PRE-FT OR POST-FT EFFECT OF GLUTAMINE WHEN CELLS ARE EXPOSED TO A FT CYCLE IN BALANCED SALT SOLUTIONS. HENCE, PROTECTION IS DUE TO ITS PRESENCE DURING THE FT-CYCLE. THE PRESENCE OF 2 ML L-GLUTAMINE IN EAGLE'S BASAL MEDIUM IS SUFFICIENT TO ACCOUNT FOR CRYOPROTECTION BY THIS MEDIUM.

Details

  • Original title: PROTECTIVE EFFECT OF L-GLUTAMINE AGAINST FREEZE-THAW DAMAGE IN MAMMALIAN CELLS.
  • Record ID : 1989-0346
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 2
  • Publication date: 1988

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