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STUDIES OF THE MECHANISMS OF MAMMALIAN CELL KILLING BY A FREEZE-THAW CYCLE: CONDITIONS THAT PREVENT CELL KILLING USING NUCLEATED FREEZING.

Author(s) : SHIER W. T.

Type of article: Article

Summary

THE AUTHOR DESCRIBES CONDITIONS THAT PREVENT KILLING OF CULTURED MAMMALIAN CELLS BY NUCLEATED FREEZING AT 249 K (-24 DEG C). OPTIMAL PROTECTION WAS OBTAINED IN ISOTONIC SOLUTIONS CONTAINING AN ORGANIC CRYOPROTECTANT SUCH AS DMSO (10%, V/V), SUCROSE FROM 50 TO 150 MILLIMOLES, AND GLUCOSE. GLYCEROL WAS ALSO AN EFFECTIVE CRYOPROTECTANT. THE DEGREE OF RESISTANCE TO KILLING BY A FREEZE-THAW CYCLE VARIED WIDELY AMONG DIFFERENT CELL LINES. STUDIES ON THE MECHANISM BY WHICH CELLS ARE PROTECTED FROM KILLING INDICATED THAT NEITHER VITRIFICATION OF THE MEDIUM NOR THE CONCENTRATING OF COMPONENTS DURING FREEZING WERE INVOLVED. ONE MODEL NOT ELIMINATED BY THE MECHANISTIC STUDIES PROPOSES THAT THE ORGANIC CRYOPROTECTANT ACTS BY FLUIDIZING MEMBRANES UNDER THE THAWING CONDITIONS, SO THAT ANY HOLES PRODUCED BY ICE CRYSTALS PROPAGATING THROUGH MEMBRANES CAN RESEAL DURING THE THAWING PROCESS.

Details

  • Original title: STUDIES OF THE MECHANISMS OF MAMMALIAN CELL KILLING BY A FREEZE-THAW CYCLE: CONDITIONS THAT PREVENT CELL KILLING USING NUCLEATED FREEZING.
  • Record ID : 1989-0345
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 2
  • Publication date: 1988

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