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Freezing tolerance of tail bud stage carp (cyprinus carpio l.) embryos: preliminary results.

Author(s) : LEVERONI-CALVI S., ZOCCARATO I., GASCO L.

Summary

Objective: evaluate the effects of prefreezing pronase treatment on freezing tolerance of tail bud carp embryos. Methodology: pronase (1 mg/ml Fish Ringer)-treated and untreated embryos were conditioned in 1 M DMSO + 0.25 M trehalose for 45 min, suckled into 0.5 ml straws and cooled at -2 deg C/min to the seeding temperature of -8 deg C, held for 15 minutes, cooled at -0.3 deg C/minute to -35 deg C, at -0.1 deg C/minute to -38 deg C, held for 2 minutes, and then plunged into liquid nitrogen. Embryos from each treatment were checked at -8 deg C before and after seeding, and at -12, -16, -20, -38, and -196 deg C. After thawing at 30 deg C and a two-step cryoprotectant removal they were evaluated for viability and cultured in Fish Ringer until hatching. Data were analysed using the ksi 2 Fisher exact test. Results: embryos survived cooling and freezing to -16 deg C, but irreversible damage occurred between -16 and -20 deg C. pronase-treated embryos showed significantly higher (P= 0.03) hatching rates when trehalose was added. The authors may speculate on a direct protection of the embryo by trehalose permeation through a fragilised chorion. These preliminary results are discussed and deeper insight in prefreezing treatments envisaged.

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Pages: p 17-23

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Details

  • Original title: Freezing tolerance of tail bud stage carp (cyprinus carpio l.) embryos: preliminary results.
  • Record ID : 1997-0973
  • Languages: English
  • Source: Refrigeration and Aquaculture.
  • Publication date: 1996/03/20
  • Document available for consultation in the library of the IIR headquarters only.

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