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Restoration of diploid genotypes by androgenesis.

Author(s) : PENMAN D. J., MYERS J. M., MCANDREW B. J.

Summary

The current inability to achieve long-term storage of fish eggs or embryos places severe limitations on gene-banking in fish. Androgenesis has potential as a means of recovering diploid genotypes from cryopreserved sperm, which could extend the application of this currently successful cryopreservation technique. By using sperm from a cryopreserved gene bank to fertilise eggs which have been genetically inactived by irradiation and inducing diploidisation of the sperm chromosomes by a variety of methods, diploid fish can be produced which have chromosomes of only paternal origin. Androgenesis has also been applied, along with gynogenesis and hormonal sex reversal, to the production of inbred lines and the manipulation of sex determination systems. Its future use in gene banking outside of the reaserch laboratory is perhaps uncertain but while the problem of cryopreserving eggs and embryos remains intractable, it offers one of the few options available.

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Pages: 469-474

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Details

  • Original title: Restoration of diploid genotypes by androgenesis.
  • Record ID : 1997-2260
  • Languages: English
  • Source: Refrigeration and Aquaculture.
  • Publication date: 1996/03/20
  • Document available for consultation in the library of the IIR headquarters only.

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