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FUNDAMENTAL STUDY ON CRYOPRESERVATION OF LIVING ORGANS USING DAPHNIA.

Author(s) : TANASAWA I., NAGATA S., IGARASHI J.

Summary

AN ATTEMPT WAS MADE TO PRESERVE LIVING WATER FLEAS (DAPHNIA MAGNA) UNDER A FROZEN STATE AT A CRYOTEMPERATURE. IN THIS STUDY, WATER FLEAS WITH 1-3 MILLIMETERS IN STATURE WERE CHOSEN AS THE SUBJECT BECAUSE THEY POSSESS VARIOUS KINDS OF TISSUES AND ORGANS. THE WATER FLEAS IN AN AQUEOUS SOLUTION OF GLYCEROL WERE FIRST COOLED AT A CONTROLLED RATE DOWN TO -30 DEG C, AND THEN RAPIDLY FROZEN IN NITROGEN VAPOR AT -196 DEG C. A RECOVERY RATIO AFTERCONTROLLED THAWING AS HIGH AS 80% IS POSSIBLE.

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Pages: 1681-1684

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Details

  • Original title: FUNDAMENTAL STUDY ON CRYOPRESERVATION OF LIVING ORGANS USING DAPHNIA.
  • Record ID : 1992-2480
  • Languages: English
  • Source: New challenges in refrigeration. Proceedings of the XVIIIth International Congress of Refrigeration, August 10-17, 1991, Montreal, Quebec, Canada.
  • Publication date: 1991/08/10
  • Document available for consultation in the library of the IIR headquarters only.

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