GRANULOCYTIC CRYOPRESERVATION: FURTHER STUDIES ON THE PATHOGENESIS OF IMPAIRED CELLULAR FUNCTION.

Author(s) : MCCARTHY D. M.

Type of article: Article

Summary

THE EFFECTS OF DIFFERENT METHODS OF PURIFYING PERIPHERAL BLOOD GRANULOCYTES ON THEIR FUNCTIONAL CAPACITY AFTER CRYOPRESERVATION HAVE BEEN STUDIED. THE STANDARD METHOD OF ISOLATION SENSITISES THEM TO DAMAGE BY SUBSEQUENT CRYOPRESERVATION. ADDITION AND REMOVAL OF DIMETHYL SULPHOXIDE WITHOUT INJURY TO GRANULOCYTES IS DESCRIBED. THE FUNCTION THAT SUFFERS MOST IS MIGRATION. THE IMPAIRMENT MAY BE DUE PARTIALLY TO UNCONTROLLED ENTRY OF CALCIUM. THE BACTERICIDAL CAPACITY IN VITRO IS NOT SO GREATLY REDUCED AND ABILITY TO REDUCE NITROBLUE TETRAZOLIUM REDOX DYE EVEN LESS SO. C.R.F.

Details

  • Original title: GRANULOCYTIC CRYOPRESERVATION: FURTHER STUDIES ON THE PATHOGENESIS OF IMPAIRED CELLULAR FUNCTION.
  • Record ID : 1985-2231
  • Languages: English
  • Source: Br. J. Haematol. - 56; 45-54; 5 tabl.; 26 ref. (voir aussi anal. 82-1052).
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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