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IMPROVED FUNCTIONAL RECOVERY OF HUMAN GRANULOCYTES AFTER CRYOPRESERVATION.

Author(s) : HILL R. S., STILL B. J., MACKINDER C. A.

Type of article: Article

Summary

HUMAN PERIPHERAL BLOOD PHAGOCYTES (90% NEUTROPHILS) WERE CRYOPRESERVED WITH EITHER 5 OR 10% DIMETHYL SULFOXIDE (DMSO) AND STORED IN THE LIQUID PHASE OF LIQUID NITROGEN. MODIFICATIONS TO THE FREEZING METHOD INCLUDED THE ELIMINATION OF DEXTRAN FROM THE FREEZING MEDIUM, ADDITION OF THE BULK OF THE DMSO AT 268K (-5 DEG C), ELIMINATION OF HEPARIN AND CENTRIFUGATION FROM ALL POSTRECONSTITUTION PROCEDURES, AND THE USE OF DEOXYRIBONUCLEASE TO MINIMIZE POST-THAW GRANULOCYTE AGGLUTINATION. SUBSTANTIAL NUMBERS OF THE CRYOPRESERVED PHAGOCYTES, AS ASSAYED BY NITROBLUE TETRAZOLIUM AND CHEMOTACTIC ACTIVITY, SHOWED COMPARABLE FUNCTIONAL ACTIVITY TO FRESH CELLS. POST-THAW CELL DIALYSIS FURTHER IMPROVED FUNCTIONAL CAPACITY ALTHOUGH PROBABLY NOT AS A CONSEQUENCE OF DMSO REMOVAL.

Details

  • Original title: IMPROVED FUNCTIONAL RECOVERY OF HUMAN GRANULOCYTES AFTER CRYOPRESERVATION.
  • Record ID : 1982-1409
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 6
  • Publication date: 1981/12

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