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EFFECT OF DIMETHYL SULPHOXIDE/HYDROXYETHYL STARCH ON GRANULOCYTE SURVIVAL (ESTIMATED BY FLUORESCENCE ASSAY) AS FUNCTION OF COOLING RATE AND FINAL TEMPERATURE OF COOLING.

Author(s) : SCHEIWE M. W., KORBER C., ENGLICH S.

Type of article: Article

Summary

ONE SERIES OF EXPERIMENTS WAS PERFORMED BY FREEZING AT DIFFERENT COOLING RATES TO 213 K (-60 DEG C), ANOTHER SERIES BY FREEZING AT 100 K/MIN TO DIFFERENT FINAL TEMPERATURES. DMSO IN COMBINATION WITH HYDROXYETHYL STARCH WAS USED AS CRYO-ADDITIVE, AND RESULTS ARE COMPARED TO EXPERIMENTAL FINDINGS PRESENTED IN EARLIER PAPERS WITHOUT ANY CRYO-ADDITIVE PRESENT. THE MAXIMUM MEAN SURVIVAL IS 68.8% AT 10 K/MIN COOLING RATE. THERE IS, HOWEVER, NO SIGNIFICANT DEPENDENCE ON THE COOLING RATE IN THE RANGE FROM 0. 5 TO 20 K/MIN. THE CRYO-ADDITIVE LARGELY AFFECTS SOLUTION EFFECT INJURY, NOT HOWEVER INTRACELLULAR ICE EFFECT INJURY.

Details

  • Original title: EFFECT OF DIMETHYL SULPHOXIDE/HYDROXYETHYL STARCH ON GRANULOCYTE SURVIVAL (ESTIMATED BY FLUORESCENCE ASSAY) AS FUNCTION OF COOLING RATE AND FINAL TEMPERATURE OF COOLING.
  • Record ID : 1985-1304
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 5
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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